Search results for "Dichroism circular spectroscopy"

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Secondary structure conformation of hydroperoxide lyase from green bell pepper, cloned in Yarrowia lipolytica, and its activity in selected media

2008

International audience; Circular dichroism (CD) spectroscopy of secondary structure conformation of the purified green bell pepper hydroperoxide lyase (HPL), cloned in the yeast Yarrowia lipolytica, was investigated. The CD spectra of HPL in iso-octane, obtained at 60 °C, in the presence of the reducing agent dithiothreitol showed dramatic increase in α-helix content. The enzyme conformation remained unchanged over a range of pH values of 5.0–7.0. Using 13-hydroperoxide of linoleic acid (13-HPOD) as substrate, the biocatalysis of HPL in organic solvent media, including chloroform, dichloromethane, hexane, iso-octane, octane and toluene, was investigated. The results indicated an increase in…

Circular dichroismChloroformbiologyStereochemistryProcess Chemistry and TechnologyDichroism circular spectroscopySubstrate (chemistry)BioengineeringYarrowiabiology.organism_classificationBiochemistryCatalysisYeastDithiothreitolHydroperoxide lyasechemistry.chemical_compoundchemistryBiocatalysisSecondary structureBiocatalysis[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyDichloromethane

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